Calibration and performance of Ig-HTS. (A) The overall success rate of tumor clonotype identification is shown per patient (left bar) and further broken down when considering number of biopsy specimens (right two bars). (B) When considering all samples assayed, fresh/frozen tumor biopsy specimens had a significantly higher rate of tumor clonotype detection compared with formalin-fixed paraffin-embedded (FFPE) specimens (Fisher exact, P = .007). (C) The success rate of tumor clonotype identification from biopsy specimens was associated with the available yield of DNA input for the reaction. An increasing success rate can be seen with increased input mass. Samples with less than minimum required DNA input (<1.5 ng) were not assayed and are therefore excluded from all panels.