EphB2 signaling is important for thrombus formation and hemostasis. (A) DiOC6-labeled control or EphB2^LacZ mouse blood was perfused over collagen-coated Vena8 Biochips and thrombus formation monitored for 10 minutes capturing images at every 30 seconds (representative images at different time points shown). (B) The fluorescence intensity of thrombi obtained from control and EphB2^LacZ mice was compared over a 10-minute period. Data represent mean (of sum intensity) ± SD (n = 4). (C) Thrombus intensity obtained in control at 10 minutes was taken as 100% and compared with EphB2^LacZ at the same time point. Data represent mean (of sum intensity) ± SD (n = 4). The P values shown in figure are as calculated by 2-way ANOVA (***P ≤ .001). (D) Laser-induced arterial thrombosis was measured in mouse cremaster muscle arterioles using intravital microscopy and the fluorescence intensity of thrombi (E) was calculated using Slidebook software (version 5.5). Representative images at different time points and cumulative trace of thrombus formation are shown. Data represent mean ± SD (n = 4 of control and EphB2^LacZ mice). The P values shown in figure are as calculated by 2-way ANOVA (F) Tail bleeding was assessed in control and EphB2^LacZ mice by dissection of 1 mm of tail tip and monitoring the time to cessation of bleeding. Data represent mean ± SD (n = 7 of control and n = 9 EphB2^LacZ mice). The P value shown was calculated using the nonparametric Mann-Whitney test. ANOVA, analysis of variance.