Macrothrombocytopenia in Dnm2^fl/flPf4-Cre mice. (A) Dnm2^fl/fl and Dnm2^fl/flPf4-Cre platelet lysates corresponding to 2 µg of protein were subjected to SDS-PAGE and probed for DNM2 and actin as indicated. (B) Blood was collected from Dnm2^fl/fl and Dnm2^fl/flPf4-Cre mice and analyzed by flow cytometry. Results are forward/side scatter dot plots and are representative of 4 independent experiments. P indicates platelets; E, erythrocytes. (C) Endogenous platelet survival measured by in vivo biotinylation. (D) High-resolution laser fluorescence spinning disk confocal microscopy imaging of GPIbα and P-selectin (CD62P) in fixed and permeabilized platelets. Bar represents 2 µm.