Immunoproliferative phenotypes in miR-142^−/− mice. (A) Splenomegaly in miR-142^−/− mice. Representative images of spleens from WT and KO mice. Spleen weights (B) and total splenocyte counts (C) in WT (n = 8) and miR-142^−/−(n = 8) animals. (D-E) Enlargement of the B-cell compartment in the KO spleen. (D) Fluorescence-activated cell sorter (FACS) analysis of lymphocytes in WT and KO spleens with anti-B220 and anti-CD19 specific antibodies. B cells (B220⁺CD19⁺) are gated, and numbers indicate the percentage of cells in the gate. (E) Total B-cell counts in WT (n = 4) and KO (n = 4) spleens. (F-G) Myeloproliferation in the KO spleen. (F) FACS analysis of myeloid (CD11b⁺) and erythroid (Ter119⁺) lineages in WT and KO spleens with anti-CD11b and anti-Ter119 specific antibodies. Numbers indicate the percentage of cells in the quadrants. (G) Total counts of CD11b⁺, CD11b⁺GR1⁺, and Ter119⁺ cells in WT (n = 4) and KO (n = 4) spleens. Results are shown as means ± standard deviation (SD) and are representative of at least 3 experiments. P values were calculated using Student t test. ***P ≤ .001; ****P ≤ .0001. SP, spleen.