Global gene expression profiling identifies genes expressed in MLL-ALL LICs but not in HSC/HPCs. (A) Transcripts of cell surface molecules over-represented in MLL-AF4 (cases 2, 3, 4, 6, and 10), MLL-AF9 (cases 14-17), and MLL-ENL (cases 18-20) LICs compared with 3 independent normal human BM HSCs. (B) Representative flow cytometry plots showing expression of CD9, CD32, CD24, and CD180 in human CD45⁺ ALL cells (MLL-AF4 case 3, MLL-AF9 case 14, and MLL-ENL case 20) and normal CB MNCs. Frequencies of cells expressing the markers are indicated. (C) Expression of CD9 and CD32 (MLL-AF4⁺ case 3), CD9 and CD24 (MLL-AF9⁺ case 16), and CD32 and CD180 (MLL-AF9⁺ case 17) in the HSC-enriched CD34⁺CD38⁻CD19⁻CD33⁻ population and LIC populations was analyzed by flow cytometry.