Figure 5
Effect of MYXV on infection, proliferation, IL-2 and IL-2Rα secretion of T cells allostimulated via MLR. To investigate whether MYXV affects the functionality of T cells in the context of allostimulation, in vitro MLR assays were carried out. PBMCs and CB from healthy donors were used to isolate MNCs. Stimulator cells were irradiated using 3000 cGy from a Cs157 and 5 × 105 of cells were plated in triplicate into 96-well plates. Responder cells were mock-treated or MYXV-treated and then 1 × 105 cells were seeded in triplicate into empty wells or in wells containing irradiated stimulator cells. After culturing for 72 hours or 96 hours, levels of infection (A-B) and proliferation (C-D) were evaluated using flow cytometry.
