Evaluation of the diabody in a model of cerebral ischemia (60 minutes) and reperfusion. Representative triphenyltetrazolium chloride–stained brain slices (A), lesion volume (B), Bederson score (C), and Grip test score (D) of mice treated with vehicle or Db (0.8 mg/kg) alone on reperfusion (60 minutes postocclusion). (E) Representative immunoblots of ipsilateral vs contralateral hemispheres. (F) Fibrin(ogen) contents in ipsilateral hemisphere (fold increase vs contralateral hemisphere) of mice treated with vehicle or diabody (0.8 mg/kg) on reperfusion (60 minutes postocclusion). All parameters were measured 24 hours after stroke onset. Data are represented as mean ± SEM (panels B,F) or median (panels C-D); n = 10-12 mice per group (panels B-D); n = 4 mice per group (panel F). *P < .05; **P < .01.