Evaluation of the thrombolytic efficacy of the diabody on fibrin-rich clots in a model of thrombin-induced MCAo. Lesion volume at 24 hours postocclusion (top) and representative T2-weighted images at 24 hours postocclusion (bottom) of mice treated with vehicle, tPA (10 mg/kg), diabody (0.8 mg/kg), or a combination of diabody (0.8 mg/kg) and tPA (10 mg/kg) at 20 minutes postocclusion (n = 6-8) (A); at 90 minutes postocclusion (n = 9-10) (B); and at 240 minutes postocclusion (n = 7-9) (C). Dotted red lines delineate stroke lesions. Data are represented as mean ± SEM. *P < .05; **P < .01. ns, not significant.