Functional assessments of hematopoietic cells isolated from leukemic mice. (A) Scheme of the colony assays and cBMTs. (B) The histogram shows the colony-forming ability of normal hematopoietic cells from leukemic and control BM. Data are represented as the mean ± SEM (n = 12; 2 independent experiments). (C) The histogram shows the single cell colony-forming ability of normal LT-HSCs from the leukemic and control BM. (D) The percentage of CD45.1⁺ donor cells in the PB of recipient mice at the indicated time points after primary cBMT. The donor cells were CD45.1⁺ BM cells. Data are represented as the mean ± SEM (n = 10-12; 2 independent experiments). (E) Short-term and long-term multilineage reconstitution capacities of donor-derived (CD45.1⁺) PB cells in primary recipients. (F) The percentage of CD45.1⁺ donor cells in the PB of recipient mice at the indicated time points after primary cBMT. Donor cells were CD45.1⁺LKS⁺CD34⁻ LT-HSCs. Data are represented as the mean ± standard deviation (SD) (n = 7). Ctrl, control. *P < .05; **P < .01; ***P < .001.