Occupancy of the KLF1 promoter during megakaryocytic differentiation of hCD34⁺ cells. Binding of transcription factors and histone modifications at KLF1 promoter were determined before (CD34) and after megakaryocytic differentiation (CD34-M) by ChIP. (A) TAL1 binding remained similar upon megakaryocytic differentiation. (B) RUNX1 binding was increased after megakaryocytic differentiation. (C) GATA1 binding was decreased upon megakaryocytic differentiation. (D) PRMT6 binding was increased after megakaryocytic differentiation. (E) WDR5 binding was decreased on megakaryocytic differentiation. (F) EZH2 binding was increased after megakaryocytic differentiation. (G) H3R2me2 was increased upon megakaryocytic differentiation. (H) H3K4me3 was decreased after megakaryocytic differentiation. (I) H3K27me3 was decreased upon megakaryocytic differentiation. (J) Knockdown of RUNX1 by 2 different shRNAs in CD34-M cells increased KLF1 mRNA amount. Quantitative PCR values of ChIP experiments are shown as percentage input. Values gathered for histone H3 modifications were normalized with a ChIP against unmodified histone H3. The P values were calculated using Student t test. *P < .05; **P < .01; ***P < .001.