Selective gene analysis of alveolar and interstitial macrophages from naïve mice. (A) Identification CD45⁺ (Lin⁻: CD3⁻ B220⁻ NK1.1⁻ Ly6C⁻ Ly6G⁻) CD64⁺MertK⁺, CD11c⁺CD11b⁻ alveolar and CD11c⁻CD11b⁺ interstitial macrophages. (B) Scatter plot of relative expression of messenger RNA (mRNA) for genes selectively expressed in favor of donor-derived AMs from alveolar (red) and interstitial (blue) macrophages isolated from naïve mice. Data shows triplicate sorts. (C) Scatter plot of relative expression of mRNA for genes selectively expressed in favor of host-derived AMs from alveolar (red) and interstitial (blue) macrophages isolated from naïve mice. Data illustrates triplicate sorts. Int Mac, interstitial macrophages.