Increased miR-486-5p levels in CML progenitor cells. (A) Heatmap showing hierarchical clustering of differentially expressed miRNA in CML and normal PBSC CD34⁺ cells (n = 5 each). (B) miR-486-5p and miR-486-3p expression by CML and PBSC CD34⁺ cells (n = 8 each) evaluated by qRT- PCR. (C) HSC, MEP, GMP, and CMP subpopulations selected from CML, normal PBSCs, and CB CD34⁺ cells were evaluated for miR-486-5p expression by qRT-PCR. (D) CD34⁺ cells cultured in myeloid (5 ng/mL SCF, 5 ng/mL IL-3 , 20 ng/mL G-CSF, and 20 ng/mL GM-CSF) and erythroid differentiation conditions (5 ng/mL SCF, 5 ng/mL IL-3, 3 U/mL EPO) were analyzed for miR-486-5p expression by qRT-PCR at days 3 and 7. Cumulative results represent the mean ± SEM. *P < .05, ***P < .001; n = 5.