Analysis of mRNA expression of peritoneal tissues. Real-time quantitative polymerase chain reaction was used to analyze the mRNA expression levels in peritoneal adhesion bands of normal (no surgery), saline-treated (vehicle), Seprafilm-treated, and APC-WT-treated mice: (A), vascular cell adhesion molecule-1; (B), intercellular adhesion molecule-1; (C), E-selectin; (D), monocyte chemotactic protein-1; (E), matrix metalloproteinas-2; (F), matrix metalloproteinas-9; (G), E-cadherin; (H), PAI-1; (I), TIMP-2. ICAM-1, intercellular adhesion molecule-1; MCP 1, monocyte chemotactic protein-1; MMP 2, matrix metalloproteinase-2; MMP 9, matrix metalloproteinase-9; VCAM-1, vascular cell adhesion molecule-1. Data are shown as mean ± SD, n = 5. *P < .05 and **P < .01 compared with saline-treated vehicle.