Effects of miR-17-92 on T-cell proliferation, activation, and survival. Purified T cells from WT or miR-17-92 KO mice (miR-17-92^fl/fl CD4-Cre⁺) on B6 background were labeled with CFSE and transferred into lethally irradiated BALB/c mice at 2 × 10⁶/mouse. Four days after cell transfer, recipient spleens were collected and subjected to cell counting and fluorescence-activated cell sorting (FACS) staining. (A-B) Percentage (as showed by representative flow figures) and absolute numbers of donor-derived (H2K^b+) CD4 and CD8 T cells on gated live cells; (C-D) representative flow figures of CFSE dilution and percentage of CFSE-diluted cells on gated donor cells; (E-F) percentage and absolute numbers of IFNγ⁺ cells on gated donor CD4 and CD8 T cells; (G) cell death along with cell division on gated donor T cells; and (H) percentage of dead cells among fast-dividing (CFSE^low) or slow-dividing (CFSE^high) on gated donor T cells. Data were shown of 1 representative mouse (A,C,E,G) or mean ± standard deviation (SD) of 4 mice per group (B,D,F,H). Two replicate experiments were performed with a total of 8 mice. *P < .05, **P < .01, ***P < .001.