Role of miR-17-92 on donor T cells in the induction of GVHD. BALB/c mice were lethally irradiated and transplanted with 5 × 106/mouse T cell–depleted marrow cells (TCD-BM, Ly5.1+) or plus purified T cells (Ly5.2+) from WT or KO mice on B6 background at the doses indicated. Recipient mice were monitored for survival (A) and body weight change (B) until 80 days after transplant. Five to seven mice were included in each group at each cell dose. Statistical analysis was performed to compare the same dose of WT and KO T cells. In separate experiments, pathology injuries (C) and scores (D) were shown 14 days post-BMT; N = 14 per group. SI, small intestine; LI, large intestine. BALB/b mice were lethally irradiated and transplanted with TCD-BM (Ly5.1+) alone or plus purified T cells at 3 × 106/mouse from WT or KO mice on B6 background. Recipient mice were monitored for survival (E) and body weight change (F) until 90 days after BMT. Data shown were pooled from 2 replicated experiments with 10 to 12 mice per group. *P < .05, **P < .01, ***P < .001.
