Prostasome/FXIIa-driven increased procoagulant activity in PC patient plasma. (A-D) Real-time thrombin generation in plasma of healthy individuals (Controls) and patients (Patients). Thrombin formation in prostasome-depleted [Patients(−)] and prostasome-reconstituted [Patients(+)] patient plasma is blotted for control; n = 9 each. (E-H) Real-time thrombin generation in normal plasma stimulated by addition of prostasomes/exosomes (250 µg/mL) from healthy female and male controls (n = 10 each) or PC patients (n = 20). Patient prostasome-triggered coagulation was measured in the absence or presence of 3F7 (100 µg/mL). (A,E) Lag time until thrombin formation starts, (B,F) time to peak thrombin, (C,G) maximum thrombin (peak thrombin), and (D,H) total thrombin (ETP). Each symbol represents an individual. Horizontal bars indicate mean values. *P < .05, **P < .01, and ***P < .001, unpaired Student t test; n.s., nonsignificant.