Gene expression profile of PAX5-JAK2⁺ patients. (A) Heatmap showing the 50 most up-/downregulated genes in cases with PAX5-JAK2⁺ leukemia (green bar) relative to the mean expression of the genes in B-cell precursor acute lymphoblastic leukemia without known sentinel alterations and with normal PAX5 status (PAX5-WT). Additionally, PAX5-ETV6⁺ (blue bar) and PAX5-C20orf112⁺ (red bar) samples are shown. (B-C) Boxplots depicting log2 fold changes of selected genes relative to the mean expression in PAX5-WT cases; the black line represents the median. Tukey-style whiskers extending to a maximum of 1.5 of the interquartile range beyond the box are depicted. Outliers beyond the range are individually plotted as circles. P values according to “limma” comparing PAX5-WT to the indicated PAX5 fusions are depicted (*P < .05, **P < .01, ***P < .001). P-C, PAX5-C20orf112; P-E, PAX5-ETV6; P-J, PAX5-JAK2; WT, PAX5 wild-type.