Microarray analysis of BMSCs with reduced TERC expression. (A) Global gene expression patterns exhibited by negative control siNC-BMSCs (NC), untreated N-BMSCs (N), and siTERC-BMSCs (T). In unsupervised hierarchical clustering, NC and N cluster together. Although there are differences between the NC and N patterns, there are clearly genes that are underrepresented (−) and overrepresented (+) in the T pattern compared with NC and N (n = 3 different N, NC and T cultures). (B) Comparison of the top under- and overrepresented genes with greater than a fourfold difference in siTERC-BMSCs reveals that CXCL12 (SDF-1) is reduced in these cells compared with siNC-BMSCs and N-BMSCs cells. (C) Further analysis of the genes showing a twofold change in siTERC-BMSCs (see supplemental Table 1), indicates a reduction in genes associated with hematopoiesis (again, CXCL12, and also Angiopoietin 1, Hepatocyte Growth Factor, and IL6. KITL (SCF) was also statistically lower in T cells than N and NC cells, although there was less than a twofold difference. The negative regulator of the Wnt, DKK1, was statistically higher in T cells than in N and NC cells (*P ≤ .05).