Figure 3
Figure 3. Detection and phenotypical characterization of βGL1-22– and LGL1-specific type II NKT cells in mice. (A) α-GalCer, βGL1-22, or LGL1 tetrameter-positive cells in the spleen of C57BL/6 mice were analyzed for the expression CD4 and CD8 expression. (B) Frequency of CD1d-βGL1-22, LGL1, or α-GalCer tetrameter-positive T cells in spleen and liver of C57BL/6 mice (n = 5). (C) Representative FACS analysis of CD3ε+ T cells costained with α-GalCer-CD1d tetramer and unloaded tetramer or βGL1-22/LGL1 tetramer from C57BL/6 mice splenocytes and liver MNCs. Results are representative of 3 experiments. (D) Flow cytometric analysis representing staining of splenocytes from WT (C57BL/6), CD1d−/−, or Jα18−/− mice with CD1d tetramers loaded with βGL1-22 or LGL1 or α-GalCer. Representative data from at least 3 experiments are shown.

Detection and phenotypical characterization of βGL1-22– and LGL1-specific type II NKT cells in mice. (A) α-GalCer, βGL1-22, or LGL1 tetrameter-positive cells in the spleen of C57BL/6 mice were analyzed for the expression CD4 and CD8 expression. (B) Frequency of CD1d-βGL1-22, LGL1, or α-GalCer tetrameter-positive T cells in spleen and liver of C57BL/6 mice (n = 5). (C) Representative FACS analysis of CD3ε+ T cells costained with α-GalCer-CD1d tetramer and unloaded tetramer or βGL1-22/LGL1 tetramer from C57BL/6 mice splenocytes and liver MNCs. Results are representative of 3 experiments. (D) Flow cytometric analysis representing staining of splenocytes from WT (C57BL/6), CD1d−/−, or Jα18−/− mice with CD1d tetramers loaded with βGL1-22 or LGL1 or α-GalCer. Representative data from at least 3 experiments are shown.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal