Enhanced effector functions in TPP2 deficient T cells. (A) Expression of the transcription factors Eomesodermin (Eomes) and T-bet in CD8⁺ T cells of the patient (P1) and a healthy adult control (healthy donor [HD]). (B) Perforin expression in CD3⁺CD4⁺ and CD3⁺CD4⁻ T cells of the patient (P1), his healthy heterozygous sister, and an adult HD control. (C) Intracellular IFN-γ and IL-17 expression in CD4⁺CD45RO⁺ T cells after stimulation with phorbol 12-myristate 13-acetate/ionomycin. The percentage of IFN-γ positive T cells in the patient was compared with a group of adult HDs (n = 24). (D) Intracellular IFN-γ of splenic CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells from TPP2-deficient knockout (KO) (n = 4) and control WT (n = 4) mice. The experiment was repeated twice with similar results.