Iron parameters and hepatic genes expression of Tfr2^BMKO mice. Iron parameters and hepatic gene expression were determined in mice 4 months after transplantation with a WT (controls) or a Tfr2^−/− (Tfr2^BMKO) BM. Three weeks before being sacrificed a group of mice was fed an ID or an IB diet. Graphed in the figure are: (A) TS; (B) splenic and (C) hepatic non-heme iron content (SIC and LIC); (D) liver messenger RNA (mRNA) expression of Tfr1, (E) bone morphogenetic protein 6 (Bmp6) and (F) Hepcidin (Hamp) relative to hypoxanthine phosphoribosyltransferase 1 (Hprt1). mRNA expression ratio was normalized to an IB control mean value of 1. Mean values of 3 to 8 animals for genotype (controls, Tfr2^BMKO) and treatment (IB, ID) are graphed. Error bars indicate standard error. Asterisks refer to a statistically significant difference. *P < .05; ***P < .005. C, controls.