Erythroid genes are expressed in the spleen of Tfr2BMKO mice. The expression of erythroid genes was analyzed in the spleen of mice 4 months after transplantation with a WT (controls) or a Tfr2−/− (Tfr2BMKO) BM. Mice were fed as in legend to Figure 1 (see text for details). In the figure are graphed: (A) splenic mRNA expression of glycophorin A (Gpa), (B) Tfr1, (C) Epor, and (D) splenic mRNA expression of Erfe relative to glyceraldehyde 3-phosphate dehydrogenase (Gapdh). mRNA expression ratio was normalized to an IB control mean value of 1. Mean values of 3 to 8 animals for genotype (controls, Tfr2BMKO) and treatment (IB, ID) are graphed. Error bars indicate standard error. Asterisks refer to a statistically significant difference. *P < .05; **P < .01; ***P < .005.