Protein expression of executioner and effector procaspases in PBMCs, normal B lymphocytes, and CLL lymphocytes. Fresh samples of normal PBMCs from peripheral blood samples obtained from healthy donors (n = 4), leukemic lymphocytes from peripheral blood samples obtained from patients with CLL (n = 9), and commercially available pure B lymphocytes (n = 3) were collected and processed for immunoblot analysis. Executioner caspases, pro- and caspase-3 protein expression (A), pro- and caspase-6 protein expression (B), pro- and caspase-7 protein expression (C) in PBMCs and CLL lymphocytes are presented. Effector caspases, pro- and caspase-8 (D) and pro- and caspase-9 (E) are shown from B cells, PBMCs, and CLL cells. Executioner caspases, pro- and caspase-3 (F), and pro- and caspase-7 (G) from normal B and CLL lymphocytes are presented. GAPDH is used as loading control for all. M denotes protein molecular weight marker. The black strip in panels D and E was used to block technically weak lanes. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.