THs acting at the membrane receptor initiate a transcriptional program. (A) A graphical heatmap representation of changes (log2 fold change) in gene expression of CUTLL1 cells after treatment with TH-AG vs CT (AG alone); n = 3 independent samples for each condition. Selected Gene Ontology categories for upregulated genes are shown on the right. (B) The top network identified by Ingenuity Pathway Analysis on mobilized genes by AG-bound hormones treated cells vs CT. (C) Validation of a select number of genes from the network shown in panel B, 24 hours posttreatment with TH-AG in immature CUTLL1 cells and mature TCL cell lines, HuT 78, and OCI-Ly12. (D) Regulation of IL-4 and VEGFB mRNA levels after 2 hours of treatment with free TH in the presence and absence of integrin αvβ3. (E) NF-κB localization by confocal microscopy of CUTLL1 cells treated or not (Control) with THs for 15 minutes. Representative photographs (scale bar, 20 μm). (F) Effect of 1 µM of NFκB inhibitor, BAY 11-7082, on TH-mediated regulation of IL-4 and VEGFB genes in CUTLL1, HuT 78, and OCI-Ly12 cells.
