Copy number variation analysis of HRS cells. (A) Representative results for all chromosomes for case 2 (top) and case 3 (bottom) are shown. HRS cells vs T-cell exon copy number changes are plotted on log2 scale. Case 2 had a relatively high frequency of copy number alterations, whereas case 3 had relatively fewer. Focal losses of the immunoglobulin genes are seen in chromosomes 14, 2, and 22 (red arrows), and gains in the TCR genes on chromosomes 7 and 14 (blue arrows). (B) Circos plot showing the segments containing copy number variations in the 10 primary cases of cHL plus the 2 cell lines sequenced. The samples correspond to cases 1 through 10 beginning at the outermost ring and followed by cell lines L1236 and L428 in the inner circle. Important oncogenes, such as REL, can be seen recurrently amplified (blue), and tumor suppressors (eg, ATM) can be seen recurrently deleted (red).
