Impaired LPS-induced ICAM1-dependent slow rolling and L- and E-selectin–dependent adhesion of neutrophils in airway postcapillary venules of P1V1 and P1V3 mice. (A) Neutrophil rolling in airway postcapillary venules of PVWT mice depends on P-selectin, slow rolling on ICAM1, and adhesion on these plus L- and E-selectin. Blocking ABs against the indicated molecules were injected into PVWT mice 15 minutes before induction of acute lung inflammation with LPS, and intravital microscopy of airway postcapillary venules was performed and assessed for rolling neutrophils (i), rolling velocity (ii), and firm adhesion (iii) from multiple videos per mouse as in Figure 3. Data are mean ± SEM of 3 to 4 mice/group. Rolling velocity is from ≥140 cells/genotype; statistics Mann-Whitney U test for genotypes with AB treatment. (B,C) LPS-induced ICAM1-dependent slow rolling and L- and E-selectin–dependent adhesion in airway postcapillary venules are impaired in P1V1 and P1V3 mice. Blocking ABs against the indicated molecules were injected into P1V1 (B) or P1V3 (C) mice before the induction of acute lung inflammation with LPS, and intravital microscopy is as in (A). Data are mean ± SEM of 3 to 4 mice/condition. Rolling velocity is from ≥140 cells/genotype; statistics unpaired Student t test for genotypes with AB treatment. Note: supplemental Figure 5 shows the same data arranged by adhesion molecule.