Phenotypic analysis and engraftment potential of human G-CSF–MPB. Phenotypic analysis of G-CSF–MPB MNCs (A-B). (A) Frequency of human CD34⁺ cells and phenotypically defined MPPs (CD34⁺CD38⁻CD45RA⁻CD90⁻CD49f⁻, abbreviated CD49f⁻) and HSCs (CD34⁺CD38⁻CD45RA⁻CD90⁺CD49f⁺Rho^lo, abbreviated CD49f⁺Rho^lo) in G-CSF-MPB MNCs obtained from 14 healthy subjects. (B) Representative flow cytometry analysis for the detection of phenotypically defined MPPs and HSCs in G-CSF-MPB MNCs; the frequency of each subpopulation is based on the parent gate shown on the top right of each plot. Using a limiting dilution transplantation approach, the frequency of HSC within the CD34⁺CD38⁻CD45RA⁻CD90⁺CD49f⁺Rho^lo subfraction was determined (C-E). (C) Schematic of the experimental design. (D) Table outlining the number of mice transplanted at each dose, the number of mice that engrafted, and the percentage of mice that failed to engraft at each respective dose. (E) Semilogarithmic plot of the frequency of long-term repopulating cells within the CD34⁺CD38⁻CD45RA⁻CD90⁺CD49f⁺Rho^lo subfraction measured by extreme limiting dilution analysis. (D) Representative flow cytometry analysis from a single NSG mouse recipient transplanted with 200 CD34⁺CD38⁻CD45RA⁻CD90⁺CD49f⁺Rho^lo cells for human cell engraftment (CD45) and lineage contribution. Analysis is representative of 3 independent experiments (n = 7 mice).