MKL1-deficient dHL-60 cells show lower content in F-actin. (A) Analysis of the F-actin content in dHL-60 MKL1 cells by flow cytometry. dHL-60 wild-type (WT) cells and dHL-60 cells carrying a scrambled or anti-MKL1 shRNA and coexpressing green fluorescent protein were mixed before fixation and staining for F-actin with Alexa Fluor 647 phalloidin. For clarity, only SCR and MKL1 populations are displayed on the flow cytometry graph. (B-C) Evaluation of the F-actin content in dHL-60 cells after stimulation with 100 nM fMLP for 1 minute in SCR and MKL1 populations, respectively. Data from 3 independent experiments were analyzed. (D) G-actin and F-actin content in dHL-60 SCR and MKL1 cells. (E-F) Analysis of the F-actin content in dHL-60 cells after stimulation with 100 nM fMLP for 1 minute in WT, MKL1, and MKL1 cells expressing mCherry G-actin. *P < .05; **P < .01; ***P < .001.