Inflammatory signaling is enriched in hemogenic endothelium. (A) runx1 GFP⁺ cells from Tg(runx1:en-GFP) transgenic embryos at 26 to 28 hpf were sorted for deep sequencing. The GO analysis of the sequencing data identified that inflammatory signaling was enriched in runx1⁺ cells. (B) Heat map analysis showed comparison of gene expression between runx1 GFP⁺ and runx1 GFP⁻ cells at 26 to 28 hpf. Multiple inflammatory signaling genes were found enriched in GFP⁺ cells. (C) cmyb⁻kdrl⁺ and cmyb⁺kdrl⁺ cells from Tg(cmyb:GFP/kdrl:mCherry) embryos were sorted at 26 to 28 hpf, and lyz⁺ cells from Tg(lyz:DsRed) embryos were sorted at 48 hpf. (D) qRT-PCR results showed that a panel of inflammatory signaling genes was enriched in cmyb⁺kdrl⁺ hemogenic endothelial cells. Each bar represents the mean ± SEM of 3 independent samples. *P < .05, **P < .01.