The deficiency of inflammatory receptors leads to HSPC defects. (A) Expression of HSPC marker runx1 was decreased in mcsfr, gcsfr, and tnfr2 morphants at 24 hpf. Black arrowheads labeled expression of runx1 in the AGM region. (B) Fluorescence-labeled hemogenic endothelial cells were decreased in Tg(cmyb:GFP/kdrl:mCherry) embryos injected with gcsfr or tnfr2 MO at 36 hpf. White arrowheads mark cmyb⁺kdrl⁺ cells in the AGM region. Each bar represents the mean ± SEM of 3 independent samples. Each sample was composed of at least 5 embryos. **P < .01. (C) qRT-PCR analysis of the NF-κB target gene il1b showed that the decreased expression of il1b in gcsfr and tnfr2 morphants at 36 hpf were rescued by coinjection of ikbaa MO. Each bar represents the mean ± SEM of 3 independent samples. *P < .05, **P < .01. (D) The decreased expression of HSPC marker runx1 in gcsfr and tnfr2 morphants were rescued by coinjection of ikbaa MO. Black arrowheads mark runx1 expression in the AGM region at 36 hpf.