CD115^lo and CD115^hi subsets of GMP-Ly6C⁺ cells are enriched for GPs and MPs, respectively. (A) CD115 expression by GMP-Ly6C⁻ and GMP-Ly6C⁺ cells was assessed by flow cytometry. (B-C) One hundred thousand CD115^lo and CD115^hi cells isolated from the GMP-Ly6C⁺ subset of CD45.2 donor mice by FACS were injected IV into CD45.1 recipient mice (nonirradiated) on day 0. Spleens were harvested from recipient mice on days 3 and 4, and splenocytes were enriched for CD45.2⁺ (donor-derived) cells by depleting CD45.1⁺ cells prior to staining to detect donor cell-derived (CD45.2⁺) neutrophils (green gates), monocytes, and macrophages (blue gates) by flow cytometry (see supplemental Figure 4 for gating strategy). CD45.2⁺CD11b⁺ splenic cells are shown. Data presented are from 1 experiment that is representative of at least 3 independent experiments. (D) Summary of surface marker expression by oligopotent GMPs and lineage-committed GPs and MPs.