The anticoagulant effects of 16:0 AC in prothrombinase assay were determined. (A) The effect of 16:0-AC on prothrombin activation by factor Xa, factor Va, and PCPS vesicles. (B) The effect of 16:0-AC on prothrombin activation by factor Xa and factor Va. (C) The effect of 16:0-AC on prothrombin activation by factor Xa and PCPS vesicles. (D) The effect of 16:0-AC on prothrombin activation by factor Xa. (E) The effect of 16:0-AC on desGla-prothrombin activation by factor Xa, factor Va, and PCPS vesicle. (F) The effect of 16:0-AC on prothrombin activation by desGla-factor Xa, factor Va, and PCPS vesicle.