ICN1 induction during EB differentiation enhances hematopoietic development. (A) Induction of ICN1 24 hours after adding Dox (0.5 μg/mL) during ESC culture is shown by real-time RT-PCR (left) and immunoblotting (right). (B) Methylcellulose (M3434) CFC counts from day 6 iICN1 EB-derived cells with indicated day(s) of ICN1 induction by Dox (0.5 μg/mL) are shown. A representative of 3 independent experiments is shown. (C) OP9 stromal cell colony counts from day 6 iICN1 EB-derived cells with indicated day(s) of ICN1 induction with Dox (0.5 μg/mL) are shown. n = 3; *P < .05 by Student t test in comparison with noninduced control.