Foxc2 induction during EB differentiation increases VE-cadherin⁺CD41⁻ population and enhances CD45⁺ cell generation from VE-cadherin⁺CD41⁻ cells. (A) Real-time RT-PCR analysis for Foxc2 expression in iFoxc2 cells 48 hours after Dox induction in comparison with KH2, 3T3, and MEF cells is shown. (B) Flow cytometric analysis of day 6 iFoxc2 EBs with or without Dox induction (0.5 μg/mL) from days 3 to 5 is shown. (C) VE-cadherin⁺CD41⁻ cells were sorted from day 6 iFoxc2 EBs with or without Dox induction (0.5 μg/mL) from days 3 to 5, and the same number of VE-cadherin⁺CD41⁻ cells was subjected to HE culture. Flow cytometric analysis of day 4 HE culture is shown. Foxc2 was not induced in HE culture. The absolute numbers of CD45⁺ cells are summarized in supplemental Table 1.