Early posttransplant Tc17 cells express altered T-cell memory markers and reduced CTL effector molecules. Phenotypic analysis of splenic CD8⁺YFP⁺ and CD8⁺YFP^neg T cells d7 posttransplant (B6.IL-17^CreRosa26^eYFP→B6D2F1). (A-D) Representative flow cytometry analysis of markers associated with (A) T-cell activation, (B) T-cell memory, (C) specialized T-cell functions and chemokine/cytokine receptor expression, and (D) CTL effector function. CD8⁺YFP⁺ (filled dark gray), CD8⁺YFP^neg (thick black), isotype (thin dashed), positive control cells (filled light gray), CD8⁺YFP⁺IL-17⁺ (thick gray), and CD8⁺YFP⁺IL-17^neg (black dotted). (E) Quantitative analysis of Granzyme B, Ly6C, IL-10R, FasL, and CD107a expression in CD8⁺ T cells (mean ± SEM, n = 5-6 mice/group; **P < .01). (C) Positive controls used: γδ TCR⁺ CD4^–CD8^– naïve T cells, NK1.1⁺ naïve NK cells, NKT tetramer⁺CD4⁺ naïve NKT-cells, MR1 tetramer⁺CD4^–CD8^– naïve T cells, and CD40L⁺CD4⁺ naïve T-cells.