Plasma VWF, but not platelet VWF, determines thrombotic occlusion rates in a carotid artery thrombosis model. (A) The carotid artery of WT (n = 8), VWF KO (n = 5), VWF PLT chimeric (n = 8), and VWF EC chimeric (n = 6) mice was exposed and a local injury was generated by topical application of a filter paper saturated with 12% FeCl₃. Carotid artery blood flow was monitored and the time needed to form an occlusive thrombus was recorded. If no occlusion occurred within 50 minutes, the experiment was ended (time to occlusion of 50 minutes). (B) Carotid artery (CA) blood flow profiles of WT (black; n = 8), VWF KO (gray; n = 5), VWF PLT chimeric (dashed gray; n = 8), and VWF EC chimeric (dashed black; n = 6) mice were recorded using a laser Doppler flow monitor. ns, not statistically significant.**P < .01; ***P < .001.