GILZ is expressed in B lymphocytes and is upregulated by GC treatment. (A) qPCR analysis of Gilz mRNA expression in WT naïve T cells and in sorted B cells subpopulation from BM (as described in supplemental Methods) (n = 3). (B) WB analysis of GILZ expression in bone marrow (BM) CD19⁺ cells or in spleen (Spl) B220⁺ cells isolated from WT and gilz KO mice. The same number of cells was loaded; WB with β-laminin antibodies served as loading control. (C) qPCR analysis of Gilz mRNA expression in WT thymus (Thy) cells and in CD19⁺ cells isolated from the BM of WT and KO mice cultured for 4 hours with or without Dex 10⁻⁷ M (n = 3). N.D., not detectable. Data in (A) and (C) were derived from 2 independent experiments and are presented relative to the expression of Actb mRNA. (D) WB analysis of GILZ expression in WT Thy cells and in BM cells from WT and gilz KO mice cultured for 4 hours with or without Dex 10⁻⁷ M. The same number of cells was loaded; WB with β-laminin antibodies served as loading control. Graphs represent mean ± standard error of the mean (SEM).