Effects of UNC0638 on H3K9Me2 and H3K9Ac chromatin occupancy at the β-globin locus and gene expression in primary human erythroblasts. (A) Integrative genome viewer screenshot at the β-globin locus in erythroblasts derived from umbilical CB or adult CD34⁺ cells that were differentiated in the presence of 0.25 μM UNC0638 or the vehicle control. ChIP-seq analysis was performed at day 11 of erythroid differentiation and after 7 days of treatment. H3K9Me2 or H3K9Ac reads are normalized to H3 reads for Mint-ChIP internal normalization, as indicated by the values on the y-axis. The tracks represent the pool of 3 biological replicates. (B) Corresponding histograms representing the normalized number of reads in the indicated region of the β-globin locus for both histone marks. H3K9Me2 or H3K9Ac reads are normalized to H3 reads for Mint-ChIP internal normalization (mean ± SD, n = 3 biological replicates). *P < .05; **P < .01; ***P < .001, relative to untreated adult erythroblasts. (C) Volcano plot illustrating changes in gene expression induced by UNC0638. RNA-seq analysis was performed in primary adult human erythroid cells at day 11 of erythroid differentiation and after 7 days of treatment with 0.25 μM UNC0638 or the vehicle control. The plot represents statistical significance vs the fold change in gene expression between the 2 conditions. Results from 3 biological replicates are shown. FDR, false discovery rate.