Platelet and neutrophil NOX2 are important for regulating the phosphorylation of AKT, ERK, and p38MAPK. (A-B) Mouse platelets and (C-D) neutrophils were treated with thrombin (0.025 U/mL) and fMLF, respectively, for 0.5, 1, and 2 minutes. (E-F) Platelets and (G-H) neutrophils were treated with 200 nM TG for 3 minutes, and 2 mM CaCl₂ was then added and incubated for 1 minute. Cells were lysed at different time points, followed by immunoblotting with equal amounts of proteins and densitometry. (A,C,E,G) Representative blots. (B,D,F,H) Quantitative graphs. Data represent the mean ± SD (n = 4-6). *P < .05, **P < .01, or ***P < .001 vs WT after Student t test.