RBC retention is reduced at concentrations of T101 that inhibit α-chain crosslinking. (A) Recalcified (10 mM, final) plasma was clotted with TF (1 pM) in the presence of increasing concentrations of T101. Clots were dissolved and analyzed by western blotting with polyclonal anti-human fibrinogen antibody. The second lane (Fgn) is unclotted plasma. (B) Normalized intensity of γ-γ dimer (open diamonds, short dashed line, n = 3) or HMW bands (open squares, long dashed line, n = 3) superimposed on normalized clot weight following clot contraction in the presence of T101 (closed circles, solid line, n = 5-7). Data are means ± SE.