Abcc4 WT and KO platelets have similar morphology. There is no difference in Abcc4 WT and KO platelet count (A) and volume (B) as determined from the complete blood count of blood. (C) Transmission electron micrographs of fixed Abcc4 WT and KO platelets. The arrow identifies the dense granules. (D) Whole-mount electron micrographs of Abcc4 WT and KO platelets with arrows (black arrowhead and red stem) indicating the dense-granules. (E) Quantification of the number of dense-granules (represented as per μm2) from the fixed platelets using transmission electron microscopy. (F) The number of dense-granules per platelet from unfixed whole-mount EMs of Abcc4 WT and KO represented as dense-granule per platelet. (G) A representative experiment of mepacrine accumulation measured by flow cytometry in Abcc4 WT and KO platelets (n = 3). Platelet count and volume data were derived from 10 to 12 mice per group and repeated twice. Transmission electron microscopy was performed with pooled platelets from 3 mice in each group. The quantification is acquired from 10 platelets. The bars in each figure represent 1 standard deviation.
