Kindlin-3 hypomorphic PMNs show impaired adhesion in vitro. (A) Densitometric quantification of kindlin-3 levels in K3+/+, K3+/n, K3n/n, and K3n/− PMNs (N = 3). GAPDH served as loading control. (B) Static adhesion of PMNs to ICAM-1 upon TNF-α stimulation (N = 4). (C) Static adhesion of PMNs to ICAM-1 upon PMA stimulation (N ≥ 5). Data are shown as mean ± SD and were statistically analyzed by Student t test. *P < .05; **P < .01; ***P < .001. n.s., not significant.