Figure 3
![Figure 3. The rate of inactivation of FXIII-A2* can occur on a physiologically relevant timescale. (A) Lineweaver-Burk plot used to generate kinetic parameters for FXIII-A2*, with data sets represented by different colors. The data point circled in pink is derived from the slope of the initial velocity in panel B (pink line). (B) The generation of FXIII-A2* cleavage products by plasmin (300 nM) at a representative FXIII-A2* concentration, determined using an ammonia release assay. (C) Standard curves of ammonia generation over time using various [FXIII-A2*]. (D) Ammonia generation over time, with varying times of FXIII-A2* digestion with plasmin. n = 3 for all experiments. Abs, absorbance.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/126/20/10.1182_blood-2015-07-650713/4/2329f3.jpeg?Expires=1735544273&Signature=GI2PMl1u~PjUQZppQghNh-xTZx7wZUYqeiZFIl2nS-g2Bd~pEnavXjGWCGM4MRuASdnI3TuDR1aFCIPCc2da-7Vh3DNaKRh6KShzAwpOugT05SxEi4QMXPXUREoMBIrCmnsjpKwMO~DC7stoeGvOrWOM4e1Ygw4ZIFy4HOpJtTEC2RkHSxtcNB~WDaeU8OT49PFH2IMq4fajUaMMy7LtydSiJAqVeg2~H7VzFzGoMfyc6BCCpyB3~HHqTWvxAEWMqlXgtSVMHT2lE~AvXeOd9fz9md-X3yR7c7W~-vtreJLn3fjLtNQJR~zq1Ti9AVLW6ogDCpGlC~VrdqhFem2Huw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The rate of inactivation of FXIII-A2* can occur on a physiologically relevant timescale. (A) Lineweaver-Burk plot used to generate kinetic parameters for FXIII-A2*, with data sets represented by different colors. The data point circled in pink is derived from the slope of the initial velocity in panel B (pink line). (B) The generation of FXIII-A2* cleavage products by plasmin (300 nM) at a representative FXIII-A2* concentration, determined using an ammonia release assay. (C) Standard curves of ammonia generation over time using various [FXIII-A2*]. (D) Ammonia generation over time, with varying times of FXIII-A2* digestion with plasmin. n = 3 for all experiments. Abs, absorbance.
