Figure 3
SPL becomes phosphorylated on serine 94 in platelets incubated with forskolin or PGI2. (A) Human platelets were incubated with 20 µM forskolin (Forsk) ± 1 µM okadaic acid (OA) as indicated. Proteins were precipitated with anti-SPL, probed with an antibody specific for pSer94 SPL and then stripped and reprobed with anti-SPL. The graph summarizes 3 experiments (mean ± SEM). (B) Identical to panel A except that the platelets were incubated with 15 µM PGI2 ± okadaic acid.
