![Figure 1. Platelet accumulation during development of APAP-induced liver injury in mice. C57BL/6J mice were treated with 300 mg/kg APAP or with saline vehicle (0 hr) IP. (A) ALT activity in plasma. (B) Concentration of platelets in blood. (C) Quantification of CD41 signal in the liver at 0.5, 2, 3, and 6 hours. Signal intensity of CD41 was normalized to the CD41 signal at time 0. (D) Representative photomicrographs of frozen liver sections labeled with anti-CD41 antibody to identify platelets; 4′,6 diamidino-2-phenylindole stain was used to identify nuclei. Platelets are pseudocolored red; nuclei are pseudocolored blue. (Original magnification ×100) #Significantly different from the saline-treated group (n = 5 mice [0 hour], 5 mice [0.5 hours], 3 mice [2 hours], 7 mice [3 hours], and 5 mice [6 hours]).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/126/15/10.1182_blood-2014-09-598656/4/1835f1.jpeg?Expires=1743232305&Signature=KHyDpJb4qoqPxX-FOAfXQv2k3fHRCACNeT5~nO0uan26niauir4RDwBcFFx1rkIvfBjrmzT8WhAiMwCknkr4GNw8bD1ZDB71PRHq96R94azCz1ibdBLCcZWqwoziRIYNlN~VSmu3ei7xbdLwVXsqsoIFoL3wUwRtUlDSYuuJpHfTsS8Kul6dz0Lmz0A6wMvD8fgwRg2~OD2zkP11UYHy8UQuQuWZVJuW3y2jg78e2~6U7OKJdJvu6Eod~WsRq6lRNBSgWfYnAqEcEq2wo5xpgiku4RCAGZKVtAtRYlGYcqiFBNXTvb-YO7i-2OjCvVQuUJbO5-zyuMIFjsIdzixLRw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Platelet accumulation during development of APAP-induced liver injury in mice. C57BL/6J mice were treated with 300 mg/kg APAP or with saline vehicle (0 hr) IP. (A) ALT activity in plasma. (B) Concentration of platelets in blood. (C) Quantification of CD41 signal in the liver at 0.5, 2, 3, and 6 hours. Signal intensity of CD41 was normalized to the CD41 signal at time 0. (D) Representative photomicrographs of frozen liver sections labeled with anti-CD41 antibody to identify platelets; 4′,6 diamidino-2-phenylindole stain was used to identify nuclei. Platelets are pseudocolored red; nuclei are pseudocolored blue. (Original magnification ×100) #Significantly different from the saline-treated group (n = 5 mice [0 hour], 5 mice [0.5 hours], 3 mice [2 hours], 7 mice [3 hours], and 5 mice [6 hours]).
