Effect of lepirudin given after APAP on hepatotoxicity. Mice were treated with 300 mg/kg APAP IP at time 0 hour. Lepirudin (1.5 mg/kg) or saline was given at 1.5, 2.5, and 4.5 hours after APAP or saline. Liver injury was determined 6 hours after APAP administration. (A) Plasma ALT activity. (B) Area of necrosis and degeneration. *Significantly different from the saline-pretreated group (N = 7 mice per group for each treatment/time).