Effect of hematopoietic cell PAR-4 deficiency on APAP-induced liver injury in mice. WT mice were irradiated and reconstituted with either WT or PAR-4^−/− bone marrow, as described in “Materials and methods.” Mice were given 250 mg/kg APAP IP. (A) ALT activity in plasma at 24 hours. (B) Area of hepatocellular necrosis at 24 hours, and (C) TAT concentration in plasma at 3 hours. No significant differences were observed. For ALT and necrosis, N = 13 mice with WT bone marrow and 9 with PAR-4^−/− bone marrow. For TAT, N = 5 mice with WT bone marrow and 4 with PAR-4^−/− bone marrow.