Figure 1
Figure 1. Impaired HSC development in the ATF4−/− FL. (A-B) Donor chimerism and RUs of E15.5 FL cells at 16 weeks after transplantation (n ≥ 11 for each genotype; 3 independent experiments; WT: ATF4+/+; KO: ATF4−/−). (C) Photographs of the embryos and FLs at E12.5. (D) Cell number per FL at E12.5 (n = 7 for each group). (E-F) Donor chimerism and RUs of E12.5 FL cells at 16 weeks after transplantation (n ≥ 14 for each group; 3 independent experiments). (G) Limiting dilution analysis of reconstitution ability in E12.5 FL. The following dose gradients were used: 0.02, 0.04, 0.08, 0.12, and 0.16 ee (n ≥ 9 for each group; 2 independent experiments). Student t test **P < .01 and ***P < .001.

Impaired HSC development in the ATF4−/− FL. (A-B) Donor chimerism and RUs of E15.5 FL cells at 16 weeks after transplantation (n ≥ 11 for each genotype; 3 independent experiments; WT: ATF4+/+; KO: ATF4−/−). (C) Photographs of the embryos and FLs at E12.5. (D) Cell number per FL at E12.5 (n = 7 for each group). (E-F) Donor chimerism and RUs of E12.5 FL cells at 16 weeks after transplantation (n ≥ 14 for each group; 3 independent experiments). (G) Limiting dilution analysis of reconstitution ability in E12.5 FL. The following dose gradients were used: 0.02, 0.04, 0.08, 0.12, and 0.16 ee (n ≥ 9 for each group; 2 independent experiments). Student t test **P < .01 and ***P < .001.

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