Uninfluenced generation of HSCs in the ATF4^−/− E11.5 AGM region. (A) Cell number per AGM region at E11.5 (n = 7 for each group). (B) Donor chimerism of E11.5 AGM region at 24 weeks after transplantation from 6 independent experiments (n ≥ 20 for each group). (C) Donor-derived multilineage chimerism in the peripheral blood of recipients at 24 weeks after transplantation (n ≥ 5 for each group). (D) Composition of myeloid (Mac-1 and/or Gr-1), B lymphoid (B220), and T lymphoid (CD3) donor cells (CD45.2) in peripheral blood at 24 weeks after transplantation. Student t test *P < .05 and **P < .01. ns, not significant.