Drastically decreased expression of Angptl3 in the ATF4^−/− FL. (A) Gene ontology (GO) terms corresponding to the downregulated genes in the stromal cells (harvested from 5 independent experiments) based on RNA-seq. (B) Expression of the genes related to HSC expansion and Angptl family genes in the E15.5 FL stromal cells from 3 independent experiments. (C) Expression of Angptl3 protein in the E15.5 FL homogenates based on enzyme-linked immunosorbent assay (ELISA) from 3 independent experiments. (D) Expression of Angptl3 mRNA in hematopoietic cells (HCs), ECs, and SCs by real-time PCR from 3 independent experiments. (E) Expression of Angptl3 mRNA in HCs, DLK^–SCF^– cells, DLK⁺SCF^– cells, and DLK⁺SCF⁺ cells (hepatoblasts) by real-time PCR from 3 independent experiments. Student t test *P < .05, **P < .01, and ***P < .001.